Standard Tests for an Olis RSM 1000 Configured for Rapid Scanning Fluorescence Emission
This document gives details on two standard tests to perform on an RSM configured for rapid scanning fluorescence emission. The first uses overhead lights which have known mercury peaks (illustrations A-F). The second is Raman scattering of distilled water.
Examples A through F are of data collected while the RSM fluorimeter was exposed to light from the overhead fluorescent lamps in the laboratory. The mercury in these lamps produces a convenient wavelength reference for establishing performance of an RSM fluorescence system. In these examples, a piece of white paper was held in front of the open port directly across from the RSM entrance slit to divert some light into the sample chamber and RSM.
CONDITIONS OF A:
|1 mm Scan Disk
||0.6 mm slit
||single beam mode
EXPLANATION OF A:
When rapid scanning the emission from a sample it is good practice to choose a low scan rate (e.g. 10 scans per second) in order to minimize noise and uncertainty in the 'live' display; once the instrument parameters are satisfactory, one can proceed to faster scan rates as appropriate for a given experiment.
Live display is smooth and stable.
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