Problems & Solutions: Olis-modernized Cary 14/17
SYMPTOM: Strange Absorbance Spectrum (Holmium Oxide)
When your instrument is set up properly and everything is functioning correctly, you can obtain the holmium oxide filter scan shown in Figure 1. Figure 2 shows the PM OUTPUT reading that will appear on a dual-channel oscilloscope when the holmium oxide filter scan shown in Figure 1 is being made. Note: the sample value is very small since the sample is absorbing the light.

Figure 1
Absorbance is calculated using the following equation: ABS = LOG [REF-DARK/ SAM-DARK]
Therefore, if the absorbance is very small and the dark value is larger due to a light leak or other problem, sam-dark becomes negative.
Since calculating the log of a negative number is not possible, the computer returns a nonsense value of 5. The return of a value of 5 is a feature we programmed into the software to alert the user that the software attempted to calculate the log of a negative number, which will occur only if there is some malfunction. Figure 2, a holmium oxide filter scan collected with a malfunctioning instrument, illustrates this software feature.

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