Olis Clarity

Olis CLARiTY series

With a CLARiTY UV/Vis, you can work in the native, natural environments for conclusions that will stand the test of time.

Living cells
Living tissue
Nanoparticles
Micro-particles
Frozen samples
Aggregating proteins, and
All light scattering samples.

Unique to the CLARiTY line:

Immunity to scatter throughout the UV/Vis spectral range
Useful with suspensions, solids, and solutions
Pathlengths of 20 or 30 centimeters using 4 or 8 mL sample holders
Available millisecond spectral scan rate

Integrating sphere and dual wavelength technologies get you close. But the CLARiTY "is an almost incredible advance on the best Britton Chance was ever able to do for turbid suspensions:

Download the PDF version

Ice crystals add scatter to these samples
(data acquired on samples shown; work done by Dr. Dima Parul)

How does it work?

In ordinary UV/Vis spectrometers, a focused beam of light shoots through the sample to an awaiting detector. If the sample is particulate, some or much of this focused measurement beam is scattered out of the detection path. The instrument can only return an 'apparent high absorbance’ reading.

In the CLARiTY, the measurement beam is a "gas of photons." Because the measurement beam is fully diffuse, it cannot be scattered further by a light scattering sample. The only way the light is changed before and after the sample is by being absorbed by the sample.

Thus, scatter does not matter, only sample absorbance does.

PowerPoint presentation
Dr. Paul Boxrud created a presentation which compares legacy UV/Vis instruments with the CLARiTY

CLARiTY sample compartment with dual beam detection

CLARiTY sample compartment shown with Olis Automatic Titrator

The Olis CLARiTY series will have extraordinary consequences in the fields of mitochondria and cancer cells, Alzheimer's, Parkinson's, lupus and other diseases, polymer and nanoparticle development, and many other contemporary studies.

See our
CLARiTY brochure

First publications employing the Olis CLARiTY!

By Robert C. Blake II, In situ spectroscopy on intact Leptospirillum ferrooxidans reveals that reduced cytochrome 579 is an intermediate in the iron respiratory chain, http://www.orau.gov/gtl2012/abstracts/blake01.pdf

By Eugene Pinkhassik, "Synergistic Co-Entrapment and Triggered Release in Hollow Nanocapsules with Uniform Nanopores." http://pubs.acs.org/doi/abs/10.1021/ja208922m

MODELS

Olis CLARiTY 1000
CLARiTY1000

The CLARiTY 1000 combines the innovative CLARiTY performance with the unbeatable speed and sensitivity of the Olis RSM 1000 rapid-scanning spectrophotometer. Choose this model when you want

Highest speed (≤ 100 scans/ second) spectral scanning
Utility in alternative measurements such as stopped-flow and CD (≤ 1000 scans/ second)
Spectral ranges of 170-540 nm, 200-800 nm, 400-1100 nm, or 800-1700 nm, including the option of all three ranges with minor hardware exchanges

"The ability to conduct direct spectrophotometric studies under noninvasive physiological conditions represents a new and powerful approach to examine the extents and rates of biological events in situ without disrupting the complexity of the live cellular environment." RC Blake II, owner of the first Olis CLARiTY 1000. See more at http://www.orau.gov/gtl2012/abstracts/blake01.pdf

Also available on non-rapid scanning models

CLARiTY 620

The CLARiTY 620 is a tiny, non-rapid scanning version of the 1000. Choose this model when

Your sample is static or very slow to change
Utility in alternative measurements such as CD
Spectral range of 170-700 nm, 400-1100 nm, or 800-1700 nm

CLARiTY 17
CLARiTY17

The CLARiTY 17 employs the world-class Cary 14/17 prism + grating monochromator. The DSPCs replace the old style cuvette holders. Choose this model when

Your sample is static or very slow to change
Utility in alternative measurements such as CD
Sub-nanometer spectral resolution, to 0.1 nm
Spectral range within 185-2600 nm

Which CLARiTY model is best for you?

	CLARiTY 1000	CLARiTY 620	CLARiTY 17
Rapid-Scanning		na	na
Scatter immunity
Pathlength enhancement
Dual beam detection
Monochromatic measuring beam
Photomultiplier tubes
UV/Vis
NIR	available	na