Olis Clarity

Olis CLARiTY series

Send us your most challenging turbid sample and let us return to you spectra that shows what is going on behind the scatter. You could get publication worthy results from this free run or simply useful information that will help you understand your system better.

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Scatter does not matter to the Olis CLARiTY series, only sample absorbance does. For the first time, one can do accurate absorbance spectroscopy on highly scattering samples such as whole red blood cells, E.coli, yeast, algae, and other "impossibly" turbid suspensions. This 100% immunity to scatter is joined by extraordinary photometric sensitivity and -- on certain models -- millisecond spectral scan rates.

The CLARiTY is a tremendous advance over prior technology alternatives including dual wavelength and integrating sphere based spectrophotometers:

✓ 100% Immunity to scatter
✓ Exceedingly high sensitivity
✓ Add this capability to most Olis spectrophotometers

How much are you missing by not having a CLARiTY?

One sample preparation, two spectrometers, two totally different results

Protein spectrum as acquired by an HP 8452 diode array. The milky looking suspension is recorded as very high absorbance with no useful structural shape. Acquisition time was 3.5 seconds.
Y axis = 0 to 2.8 AU

Click on the image to Enlarge

The same protein suspension at the same concentration was then scanned by the Olis CLARiTY 1000A spectrophotometer. Because scatter does not matter to the CLARiTY, the correct absorbance and a very descript (and accurate) spectral shape can be acquired. Data acquisition time was 5 seconds.
Y axis = 0.002 to 0.015 AU

Click on the image to Enlarge

"Wow! I was not expecting to see a UV maximum at 265 and 295nm!! Maybe from exciton coupling between the phenylalanine aromatic electronic transitions. Definitely not something we could see with out UV."
-Anil Kumar Mehta, research associate with David Lynn at Emory University
See the results which Professor Lynn referred to as "spectacular": Lynn protein spectra

Algae samples on an Olis CLARiTY 1000 at two different pHs

Clarity algae
Click on the image to Enlarge

Data collected on whole algae cells of "armoured" and unarmoured versions of the same organism. The calcium deposits came off when the pH was changed by adding CO2 [2 should be subscript]. These two data sets were collected on an OLIS CLARiTY 1000A during installation at the University of Essex. Collection time was 5 seconds per scan.

Olis CLARiTY: Novel detection chamber

In a normal spectrophotomer, if a focused beam of light passes through a turbid solution, most of it is scattered in all directions, and only a tiny fraction of the original beam makes it into the detector. Thus, the detector can only report that most of the light is not there, as if it were absorbed. Therefore, the answer is returned as 'apparent high absorbance,' but this is inaccurate.

In the CLARiTY, the beam of light is a "gas of photons." The beam is not focused, but is instead fully diffuse. Because it is already fully diffuse, it cannot be scattered further by a light scattering sample.

We call the sample holder a "DeSa Suspension Presentation Chamber" or DSPC. Early DSPC designs are spherical with long chimneys, allowing entry of reactants manually or with a titrator or other device.

The 4 mL DSPC does not need to be full. The gas of photons is everywhere within the DSPC, so we can say that "all of the light is seeing all of the sample all of the time." This is true whether the DSPC is partially or completely filled. The DSPC is flooded with the gas of photons, and the photons are bouncing continuously within every position of the DSPC until fully absorbed or exiting and being detected by the photomultiplier tube.

DeSa Suspension Presentation Chamber

In the CLARiTY, the 'cuvette' is enclosed within a highly reflective material. The measurement beam -- the gas of photons – bounces off of the walls of the cuvette over and over and over again, enhancing the effective pathlength of many centimeters from a 4 mL sample. In the 4 mL DSPC, the effective pathlength is 20 centimeters! Very little sample is necessary for great results. One can get the correct absorbance using just a puddle of solution and leave room for the addition of reactants, for instance.

DSPC with titrator

The Olis CLARiTY series will have extraordinary consequences in the fields of mitochondria and cancer cells, Alzheimer's, Parkinson's, lupus and other diseases, polymer and nanoparticle development, and many other contemporary studies.

See our
CLARiTY brochure

Click to View See 3 experiments
performed with CLARiTY

or download the PDF version

First publications employing the Olis CLARiTY!

By Robert C. Blake II, In situ spectroscopy on intact Leptospirillum ferrooxidans reveals that reduced cytochrome 579 is an intermediate in the iron respiratory chain, http://www.orau.gov/gtl2012/abstracts/blake01.pdf

By Eugene Pinkhassik, "Synergistic Co-Entrapment and Triggered Release in Hollow Nanocapsules with Uniform Nanopores." http://pubs.acs.org/doi/abs/10.1021/ja208922m

MODELS

Olis CLARiTY 1000
CLARiTY1000

The CLARiTY 1000 combines the innovative CLARiTY performance with the unbeatable speed and sensitivity of the Olis RSM 1000 rapid-scanning spectrophotometer. Choose this model when you want

Highest speed (≤ 100 scans/ second) spectral scanning
Utility in alternative measurements such as stopped-flow and CD (≤ 1000 scans/ second)
Spectral ranges of 170-540 nm, 200-800 nm, 400-1100 nm, or 800-1700 nm, including the option of all three ranges with minor hardware exchanges

"The ability to conduct direct spectrophotometric studies under noninvasive physiological conditions represents a new and powerful approach to examine the extents and rates of biological events in situ without disrupting the complexity of the live cellular environment." RC Blake II, owner of the first Olis CLARiTY 1000. See more at http://www.orau.gov/gtl2012/abstracts/blake01.pdf

Also available on non-rapid scanning models

CLARiTY 620

The CLARiTY 620 is a tiny, non-rapid scanning version of the 1000. Choose this model when

Your sample is static or very slow to change
Utility in alternative measurements such as CD
Spectral range of 170-700 nm, 400-1100 nm, or 800-1700 nm

CLARiTY 17
CLARiTY17

The CLARiTY 17 employs the world-class Cary 14/17 prism + grating monochromator. The DSPCs replace the old style cuvette holders. Choose this model when

Your sample is static or very slow to change
Utility in alternative measurements such as CD
Sub-nanometer spectral resolution, to 0.1 nm
Spectral range within 185-2600 nm

Which CLARiTY model is best for you?

	CLARiTY 1000	CLARiTY 620	CLARiTY 17
Rapid-Scanning		na	na
Scatter immunity
Pathlength enhancement
Dual beam detection
Monochromatic measuring beam
Photomultiplier tubes
UV/Vis
NIR	available	na